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. 2009:3357–3555. doi: 10.1007/978-3-540-29678-2_18

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© Springer-Verlag GmbH Berlin Heidelberg 2009

This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

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Respiratory Network Analysis, Functional Imaging. Figure 4 — Targeted recording from acutely fluorescence-tagged preBötC neurons. (a, b) tetramethylrhodamine conjugated to substance P (TMR-SP) labeling in preBötC neurons with different phenotypic properties. Infrared differential interference contrast (IR-DIC) and epifluorescence images are shown in left columns, with corresponding intracellular traces to the right. (a) TMR-SP⁻ early inspiratory neuron with silent interburst intervals. (b) TMR-SP⁺ early inspiratory neuron with tonic low-frequency spiking properties. Scale bar (25 µm) applies to all images in (a, b). (c, e) simultaneous measurements of inspiratory activity and TMR-SP labeling in preBötC neurons. (a) fluo-4 image shows a peak acquisition of Ca²⁺ labeling; TMR-SP image shows TMR-SP⁺ cells in the same region. Scale bar: 50 µm. (d) changes in fluorescence intensity from regions of interest (ROIs) indicated by numerals in (c), plotted with synchronized XII activity (reproduced from [6] with permission).