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. 2007 Dec 17;582(2):221–228. doi: 10.1016/j.febslet.2007.12.004

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FEBS Letters 582 (2008) 1873-3468 © 2015 Federation of European Biochemical Societies

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

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figure image — Immobilization and purification of DBtag‐fusion proteins on agarose/DNA microplate. (A) Schematic diagram showing immobilized DBtag‐fusion proteins on the microplate before and after washing. (B) Only DBtag‐GFP was immobilized on the microplate, while washing removed all other tagged GFPs and the untagged GFP. (C) DBtag‐GFP and RFP (untagged) were mixed together and the mixture was spotted on the microplate. The DBtag‐GFP was immobilized on the microplate, while the RFP was washed out and no RFP fluorescence was observed. (D) GFP (untagged, upper spot) and DBtag‐GFP (lower spot) were spotted on the microplate. The DBtag‐GFP was immobilized on the microplate by 5% of detergent treatment (5% Tween‐20 and 5% NP40 panels), while the GFP was washed out and no GFP fluorescence was observed.