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. 2019 Nov 2;594(4):678–694. doi: 10.1002/1873-3468.13642

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© 2019 Federation of European Biochemical Societies

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

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Determination of optimal MTase reaction conditions for VEEV (●) and CHIKV nsP1 (■). The optimal MTase reaction conditions were determined by individually varying (A) temperature, (B) pH, (C) KCl concentration, and (D) magnesium ion concentration, while the other three parameters were kept at optimal levels. Activity was calculated as an increase in the corrected peak area of SAH in the CE electropherogram. For each parameter, the optimal activity observed was taken as 100% and the relative activity was calculated at other conditions. Data points represent mean values and error bars are standard deviations from duplicate experiments.