Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Nov 15;273(24):5574–5588. doi: 10.1111/j.1742-4658.2006.05547.x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

PMC Copyright notice

Chromatographic purification of midgut aminopeptidase from A. pisum. (A) Chromatography on Mono Q equilibrated with 20 mm Tris/HCl buffer (pH 7.0)/0.1% Triton X‐100. Elution was accomplished with a gradient of 0–600 mm NaCl gradient in the same Tris buffer (substrate used LeupNA). (B) SDS/PAGE of samples obtained after the steps from A. pisum APN purification (12% polyacrylamide slab gels, silver staining). Lane 1, midgut homogenate; lane 2, Triton X‐100‐released proteins from midgut cell membranes; lane 3, Mono Q eluate (purified aminopeptidase). (C) Glycoprotein detection (Dig Glycan detection kit), after western blots of proteins. Lane 4, midgut homogenate; lane 5, purified APN; lane 6, purified with the differentiation kit with the mannose‐specific lectin Galanthus nivalis agglutinin.