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Properties of purified midgut APN from A. pisum. (A) Effect of pH on enzyme activity (optimal pH 7.0 ± 0.5). Buffers used: 100 mm sodium phosphate buffer (pH 5–7) and 100 mm Tris/HCl buffer (pH 7–9.5). (B) Isoelectric focusing (pI 8.4 ± 0.2). (C) Density‐gradient centrifugation. Hb, Haemoglobin; Ct, catalase. Molecular mass was calculated as 130 kDa. (D) Arrhenius plot. Activation energy was determined as E a = 42.2 kJ·mol−1.
