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. 2008 Jul 9;275(16):4152–4163. doi: 10.1111/j.1742-4658.2008.06564.x

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© 2008 The Authors Journal compilation © 2008 FEBS

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

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Translocation of the SARS‐CoV N protein to cytoplasmic granules can be induced by cell stress and modulated by phosphorylation. (A) Expression vector encoding FLAG‐tagged full‐length (N), RS motif‐deleted (NΔRS), two serine‐to‐alanine mutants (N‐6A and N‐14A), N‐terminal‐half (N^NT) or C‐terminal‐half (N^CT) N protein was transiently transfected into HeLa cells. Upper panel: representative fluorescence images. Lower panel: percentage of granule‐positive cells; approximately 100 transfected cells were counted for each protein. (B) HeLa cells transiently expressing HA‐tagged N or NΔRS or coexpressing HA‐N and GFP‐TIA‐1 were mock treated (−) or treated (+) with 0.5 mm arsenite for 1 h. Double immunofluorescence was performed using anti‐HA and anti‐PABP. A merged image is shown in the right‐hand panel.