Figure 5.

Structural representations of the three glycans investigated in this work. Shown below is the HSQC spectrum of the holo‐CRD (0.4 0.7 mm) at pH 4.2 in the absence (black) and presence (green) of 5 mm Man₃ (12.5‐fold molar excess), 10 mm GlcNAc₂Man₃ (14‐fold molar excess; red), and 10 mm GlcNAc₃ (14‐fold molar excess; blue). The addition of Man₃ to the CRD at low pH yielded no changes in the CRD spectrum, suggesting that Man binding to the CRD is pH‐dependent. Some small perturbations were observed upon addition of GlcNAc₂Man₃ and GlcNAc₃ at low pH, and suggest that binding of the CRD to the GlcNAc moiety may not be completely abolished at low pH.