Figure 6.

Elution of the CRD from a Man–Sepharose column with EDTA or low‐pH buffer. (A) The CRD was eluted from the Man–Sepharose column with 5 mm EDTA in 20 mm Hepes buffer (pH 7.8) and 150 mm NaCl. The majority of the protein was eluted in fraction 2, although small amounts of protein were also detected in other fractions, as well as in the wash (W). (B) In an equivalent experiment, the CRD was also eluted from the column with an EDTA‐free buffer (same as above) adjusted to pH 4.2. The majority of the protein was eluted in fraction 3, confirming that the CRD is capable of releasing ligand at low pH. As observed in (A), there appeared to be a small amount of residual protein in subsequent fractions, as well as in the wash (W).