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Recombinant expression of PILS‐AP in insect cells. Aliquots (10 µg protein per lane) of the Sf9 cell fractions were separated by SDS/PAGE, and recombinant PILS‐AP was detected using antibodies directed against the FLAG‐epitope. The molecular mass (kDa) of standard proteins is indicated on the left. H, homogenate; P1, 3000 g pellet; S, high‐speed supernatant; P2, high‐speed pellet.
