Figure 5.

Substrate specificity. The aminopeptidase activities towards the indicated aminoacyl‐β‐naphthylamide derivatives are given. Control and PILS‐AP‐baculovirus infected Sf9 cells were homogenized, and aliquots of the high‐speed supernatants (5 µg protein) were incubated at 30 °C for 30 min with the aminoacyl‐β‐naphthylamide derivatives (100 µm final concentration) in the presence (+ P) or absence of puromycin (5 µm). Liberated β‐naphthylamide was determined fluorimetrically. The activities towards Ala‐, Arg‐, Asn‐, Asp‐, Gln‐, Glu‐, Gly‐, His‐, Lys‐, Pro‐, Ser‐ and Trp‐β‐naphthylamide were below 0.1 pmol liberated β‐naphthylamide·min⁻¹·µg⁻¹.