Table 1.

Effect of various protease inhibitors on PILS‐AP activity. The indicated substances were preincubated with the enzyme on ice for 5 min and the reaction was started by adding Leu‐β‐naphthylamide to 0.1 mm.

Chemical (preincubation)	Concentration (µm)	Inhibition (%)
None		0
Di‐isopropyl fluorophosphate	2000	7
2‐Iodoacetamide	2000	5
Dithiothreitol	100   1000 10 000	2 11 61
1,10‐Phenanthroline	100    200	74 88
4,7‐Phenanthroline	100    200	23 37
1,7‐Phenanthroline	100    200	11 21
EDTA (5 min, 4 °C) EDTA (20 h, 4 °C) EDTA (48 h, 4 °C) EDTA (240 h, 4 °C)	1000   1000   1000   1000	0 19 49 96
Phosphoramidon	10	0
l‐Leucinethiol	0.1      1.0	85 97
Leucinol	100	0
Puromycin	1.0      5.0	0  0
Actinonin	1.0	23
Amastatin	1.0	44
Bestatin	1.0	11