Table 3.
Comparison of DHA production of each cycle in repeated fed-batch culture of four cycles
| Repeated fed-batch | Fermentation time (h) | Dry cell weight (g/L) | DHA (g/L) | DHA productivity (mg/L/h) | Fatty acid composition (%)a | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| C12:0 | C14:0 | C16:0 | C18:0 | C18:1 | C22:6 | |||||
| Cycle 1 | 120 | 46.80 ± 0.87 | 6.18 ± 0.01 | 51.54 ± 0.06 | 3.08 ± 0.36 | 14.28 ± 1.13 | 18.77 ± 0.56 | 2.58 ± 0.36 | 12.18 ± 0.18 | 49.12 ± 2.04 |
| Cycle 2 | 120 | 45.98 ± 0.24 | 6.05 ± 0.03 | 50.43 ± 0.22 | 3.74 ± 0.26 | 14.00 ± 0.20 | 19.45 ± 0.18 | 2.19 ± 0.03 | 11.61 ± 0.23 | 49.02 ± 0.50 |
| Cycle 3 | 120 | 46.48 ± 0.29 | 6.11 ± 0.06 | 50.94 ± 0.54 | 3.88 ± 0.04 | 12.95 ± 0.84 | 19.16 ± 0.93 | 2.85 ± 0.36 | 12.09 ± 0.36 | 49.06 ± 1.48 |
| Cycle 4 | 120 | 44.76 ± 0.08* | 5.88 ± 0.08 | 48.97 ± 0.64 | 3.58 ± 0.35 | 12.82 ± 0.58 | 20.25 ± 0.38 | 3.43 ± 0.73 | 11.40 ± 0.34 | 48.53 ± 0.49 |
aLauric acid (C12:0). Myristic acid (C14:0). Palmitic acid (C16:0). Stearic acid (C18:0). Oleic acid (C18:1). Docosahexaenoic acid (C22:6)
* Significant differences between cycle 4 and cycle 1 evaluated by a statistical Student’s T-test (*p < 0.05)