Fig. 5.

MHC-II and αSYN are located in LAMP-1 positive vesicles. Immunostainings showed that the LAMP-1 expression pattern was altered in αSYN-F exposed astrocytes, but not in astrocytes exposed to αSYN-M or IFNγ only (a). The αSYN deposits in αSYN-F exposed astrocytes are clearly localized within LAMP-1 positive vesicles (b). Close-up of white rectangles in b are shown in b’ and b”. Confocal analysis confirmed the localization of αSYN within the lysosomal compartments (c). Stainings for MHC-II and LAMP-1 in astrocytes, exposed to unlabeled αSYN-F, confirmed that also MHC-II is located in the LAMP-1 positive vesicles (d).Close-up of the white rectangle in d is shown in d’. Scale bars: a, b = 20 μm; c = 2 μm, d = 5 μm