Fig. 6.

Aggregated αSYN and MHC-II are transported between astrocytes via tunneling nanotubes. Immunostainings and confocal imaging demonstrated transfer of MHC-II between the astrocytes via WGA-labeled tunneling nanotubes (a). A close-up of the tunneling nanotube region (white rectangle) is shown in a’. The various z-planes (z1–z6) for the WGA-staining and MHC-II-staining are shown separately in black and white. Aggregates of αSYN and MHC-II could be detected in tunneling nanotubes connecting two astrocytes (b). A close-up of the tunneling nanotube region (white rectangle) is shown in b’. The various z-planes (z1–z4) for the αSYN and MHC-II-staining are shown separately in black and white. Scale bars: (a) = 20 μm and (b) = 10 μm