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. 2020 Apr 17;21:30. doi: 10.1186/s12860-020-00274-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — TMIGD1 is expressed by renal epithelial cells with distinct subcellular locations. a Immunofluorescence analysis of murine kidney sections with TMIGD1 antibodies (#HPA021946). The insets (Inset 1, Inset 2) show magnifications of the areas demarcated with rectangles (I-1, I-2) in the left panel. Note that TMIGD1 is enriched at cell-cell contacts in some tubular epithelial cells but is localized in the cytoplasm in others. Scale bar: 100 μm. b Immunohistochemistry on murine kidney sections with antibodies against TMIGD1 (#HPA021946). Control stainings (bottom panel) were performed in the absence of the primary antibodies. Note that TMIGD1 is expressed by tubular epithelial cells but is absent from glomerular cells. Scale bars: 50 μm. c Immunofluorescence analysis of murine kidney sections. Kidney sections were stained with FITC-conjugated PHA-E lectin and with anti-TMIGD1 antibodies (#HPA021946). Note that all TMIGD1-positive structures are positive for PHA-E (marked by asterisks) suggesting preferential expression of TMIGD1 by epithelial cells of proximal tubules. Scale bar: 100 μm