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. 2020 Apr 17;21:30. doi: 10.1186/s12860-020-00274-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Ectopic Myc-SYNJ2BP recruits TMIGD1 to mitochondria in a PDZ domain-dependent manner. MDCKII-TetOFF cells stably expressing either Flag-TMIGD1 WT (a), Flag-ΔD1-TMIGD1 (b), or Flag-ΔD1-TMIGD1/Δ5 (c) were transiently transfected with Myc-SYNJ2BP. Cells were stained with antibodies against Myc-SYNJ2BP (anti Myc, SantaCruz #sc-789G), Flag-TMIGD1 (anti Flag, Sigma #F7425), and either endogenous β-catenin to visualize cell-cell contacts or with mitotracker to visualize mitochondria, as indicated. The bar graphs in each panel show quantifications of Flag-TMIGD1 localization in Myc-SYNJ2BP-expressing cells (TMIGD1 co-localization with Myc-SYNJ2BP in mitochondria vs TMIGD1 localization in the cytoplasm (panel a), or TMIGD1 co-localization with Myc-SYNJ2BP in mitochondria vs TMIGD1 localization at β-catenin-positive cell contacts (ΔD1-TMIGD1 and ΔD1-TMIGD1/Δ5, panels b and c). Data are indicated as the fraction of Myc-SYNJ2BP-positive cells that are positive for TMIGD1 at mitochondria vs cytoplasm (a) or cell contacts (b, c). Data were obtained from at least three independent experiments with at least 50 cells analyzed. Error bars indicate standard deviations between experiments. Scale bars: 10 μm