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. 2020 Apr 16;11:155. doi: 10.1186/s13287-020-01652-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Construction and identification of the ORF-DPYSL2 lentivirus. a Information of the EX-Rn10192-Lv201recombinant. b Enzyme digestion products of EX-Rn10192-Lv201recombinant, separated by 3% agarosegel electrophoresis. Lane 1, 4, and 5: DNA Ladder. Lane 2: EX-Rn10192-Lv201 plasmid. Lane 3: EX-Rn10192-Lv201 plasmid digested by NspVandXhoI. c Represent NSCs pictures taken by inversed fluorescent microscope before and at 3 and 7 days post transfection. Green: eGFP positive cells. Scale bar = 50 μm. Nor, normal group, neural basal medium addition; eGFP, eGFP group, eGFP-lentivirus transfection; DPYSL2, DPYSL2 group, DPYSL2-ORF vector transfection