Table 1.
Primer | Supplier | Sequence 5′-3´ | |
---|---|---|---|
Forward | Reverse | ||
GADPH | Sigma-Adrich, Steinheim, Germany | TGTAGTTGAGGTCAATGAAGGG | ACATCGCTCAGACACCATG |
STAT-3 | IDT, Leuven, Belgium | AGGCATTTGGCATCTGACAG | TGCTTCCCTGATTGTGACTG |
TNF-α | IDT | TCAGCTTGAGGGTTTGCTAC | TGCACTTTGGAGTGATCGG |
IL-1β | Qiagen, Hiden, Germany | Not provided by the manufacturer | Not provided by the manufacturer |
IL-1α | IDT | AGTTCTTAGTGCCGTGAGTTTC | GTGACTGCCCAAGATGAAGA |
IL-6 | IDT | GCCCCACACAGACAGCCACTCACC | TGCCTCTTTGCTGCTTTCACACAT |
IL-23 | IDT | GGCGCAGAGCCAGCCAGATT | ACCCTCAGGCTGCAGGAGTTGG |
GAPDH indicates glyceraldehyde-3-phosphate dehydrogenase; IL, interleukin; STAT, signal transducer and activator of transcription; TNF, tumor necrosis factor