Figure 5.
5mC accounts for only two-third of the variance in cytosine modifications in human cerebellum. For blood (a), cerebellum (b), and saliva (c), βoxBS (blue lines, 5mC) and βBS (red lines, 5mC + 5hmC) were plotted from 5 kb upstream of the TSS (5 kb+) to the TSS, across the intragenic region to the TES, and 5 kb downstream of the TES (5 kb−) for >13 695 genes containing detectable 5mC. For blood and saliva, the vast majority of cytosine modification signal from bisulfite-converted DNA is that of 5mC, with the gene body region in saliva samples showing slightly more contribution from 5hmC. For cerebellum, however, 5mC contributes to only two-third of the cytosine modification signals, not only in the gene body but also across the entire representative genome.