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. 2020 Jan 8;6(2):113–123. doi: 10.1002/cjp2.156

Figure 2.

Figure 2

CDKN2A transcript levels detected despite p16 immunonegativity in chordoma samples and cell lines and lack of correlation to miRNA expression. (A) Expression of CDKN2A transcript assessed in 10 chordoma samples by RNA‐sequencing. FPKM, fragments per kilobase of transcript per million mapped reads. (B) Expression of CDKN2A transcript assessed in 22 chordoma samples by qPCR. (C) Expression of CDKN2A transcript in chordoma cell lines by qPCR: p16‐negative with homozygous deletion (UCH1, UCH2, MUG‐Chor), p16 negative and monosomic (UCH7, UM‐Chor, UCH11), p16‐positive and monosomic (JHC7). (D–F) Expression of miRNA‐10, –24 and –125 in chordoma cases, as assessed by FISH, and showing positivity or negativity for p16 protein expression by IHC. Cases showing monosomic or disomic CDKN2A copy number were combined in this analysis.