Table 2.

p16 IHC and CDKN2A FISH of multiple samples from 39 patients

Study unique ID	Anatomical site	Age at dx	Primary	LR1	LR2	LR3	LR4	LR5	Met	Events per patient	IHC over time	FISH over time
5	Mobile spine	53								2	Pos > Neg	Mono > Mono
20	Mobile spine	68								2	Pos > Pos	Mono > Diso
22	Sacrum/coccyx	63								2	Neg > Neg	NI
23	Sacrum/coccyx	72								2	Pos > Pos	NI
39	Sacrum/coccyx	‐								2	Neg > Pos	Disomy > Disomy
42	Sacrum/coccyx	58								2	Neg > Neg	Del Homo > Del Homo
54	Sacrum/coccyx	43								2	Pos > Neg	Del Het > Del Het
56	Sacrum/coccyx	61								2	Neg > Neg	Del Homo > Del Homo
61	Sacrum/coccyx	58								4	Neg > Neg	NI
67	Sacrum/coccyx	54								2	Pos > Pos	Del Het > Del Het
88	Sacrum/coccyx	67								2	Neg > Pos	Mono > Mono
90	Sacrum/coccyx	67								2	Neg > Neg	Del Het > Del Het
95	Sacrum/coccyx	63								3	Neg > Neg	Mono > Mono
131	Sacrum/coccyx	69								2	Pos > Pos	NI
134	Sacrum/coccyx	65								2	NI	NI
135	Sacrum/coccyx	65								2	Pos > Pos	NI
136	Sacrum/coccyx	73								2	Neg > Neg	NI
137	Sacrum/coccyx	69								2	Neg > Neg	NI
138	Sacrum/coccyx	61								3	Neg > Neg	Del Het > Del Het
143	Sacrum/coccyx	‐								6	Pos > Pos	Poly > Poly
153	Sacrum/coccyx	59								2	Pos > Pos	Mono > Mono
157	Sacrum/coccyx	53								2	Pos > Pos	Mono > Poly
162	Skull base	43								3	Pos > Pos	Mono > Poly
165	Mobile spine	49								2	Pos > Pos	NI
166	Sacrum/coccyx	63								2	Neg > Neg	Mono > Mono
168	Skull base	47								3	NI	Diso > Mono
173	Skull base	19								2	NI	NI
174	Skull base	35								2	Pos > Pos	NI
175	Sacrum/coccyx	35								3	Neg > Neg	Del Het > Del Het
177	Skull base	37								3	Pos > Pos	Poly > Poly
182	Skull base	65								2	Pos > Pos	Diso > Diso
209	Skull base	40								4	Pos > Pos	Diso > Poly
211	Skull base	63								3	Neg > Pos	Del Het > Del Het
212	Skull base	46								4	Pos > Neg	Diso > Del het > Del homo
213	Skull base	60								3	Pos > Neg	Diso > Mono
214	Mobile spine	57								2	Pos > Neg	Diso > Del homo
217	Skull base	31								3	Neg > Pos	Mono > Mono
218	Vertebra	53								5	Neg > Neg	Del Homo > Del Homo
219	Mobile spine	48								3	Neg > Pos	Del Homo > Del Het

Blue, samples analysed. p16 IHC was NI for three patients, leaving 36 patients available for analysis. Del Het, Heterozygous deletion; Del Mono, Homozygous deletion; Diso, disomy; Dx, diagnosis; LR1–LR5, first to fifth local recurrence; Met, metastasis; Mono, monosomy; Neg, p16‐negative; NI, non‐informative; Poly, polysomy; Pos, p16 positive.