Figure 5.

β2AR stimulation upregulated the expression of Aird5a, resulting in IL‐6 mRNA induction. (A) CFs were treated with ISO (10 μmol/L) for indicated times. The expression of Arid5a mRNA was measured by real‐time RT‐PCR. Results are shown as mean ± SEM (n = 6). **P < .01 vs 0 h by Dunnett test. (B‐F) CFs were treated with the indicated concentrations of ISO (B), SAL (C), CL‐316243 (D), FSK (E), or dBcAMP (F) for 1 h. The expression of Arid5a mRNA was measured by real‐time RT‐PCR. Results are shown as mean ± SEM (B: n = 8, C‐F: n = 6). *P < .05, **P < .01 vs vehicle by Dunnett test. G) CFs were isolated from WT mice or Arid5a KO mice and treated with or without SAL (1 μmol/L) for indicated times. The expression of IL‐6 and IL‐1β mRNA in CFs was measured by real‐time RT‐PCR. Results are shown as mean ± SEM (n = 6). **P < .01 vs WT by Student's t test. H) CFs were isolated from WT mice or Arid5a KO mice and treated with or without SAL (1 μmol/L) for 24 h. IL‐6 protein, secreted by CFs in culture medium, was measured by ELISA. Results are shown as mean ± SEM (n = 6). **P < .01 by Tukey‐Kramer test