Table 1. Top 20 samples with Delftia DNA identified via qPCR targeting of unique “gold gene”.

DNA Sample number	Delftia gold gene count	Latitude	Longitude	Sample type	Location	Description
15-1	113,191	35.78593062	−78.66805315	Swab	Poe Hall	Water fountain
7-1	17,294	35.78472956	−78.67292404	Swab	Owen Residence Hall	None provided
24-1	14,167	35.78822065	−78.67522672	Swab	University Towers	Parking deck drains
1-3	12,041	35.78654	−78.671737	Swab	Williams Hall	Bathroom sink
17-3	9,493	35.78068018	−78.67308866	Swab	Wood Residence Hall	Sink drain
23-2	8,780	35.78468	−78.666723	Swab	SAS building	The girls bathroom sink on the first floor of SAS building, middle sink
33-2	5,789	35.78744498	−78.67013454	Swab	D.H. Hill Jr. Library	3rd floor women’s bathroom sink
12-2	5,095	35.785385	−78.673091	Swab	Metcalf bathroom	Bathroom sink
26-1	5,095	35.74477072	−78.68757963	Swab	Campus Crossing	Apartment complex
9-1	4,612	35.78795303	−78.67699295	Swab	Valentine Commons	Kitchen sink
44-4	4,356	35.78670028	−78.67463044	Soil	Fence on Dan Allen Dr.	Chilly (56 F), drier soil, live organisms present
25-2	2,961	35.7861221	−78.66352558	Swab	NCSU bell tower, main campus	Wild Card sample-seat located on NCSU bell tower
45-4	2,317	35.78753054	−78.67083426	Soil	Atrium	Trash bins next to the vending machines
24-2	1,971	35.78822065	−78.67522672	Swab	University Towers	Drain
15-2	1,873	35.785982	−78.677831	Swab	Lee Hall	Suite 807 Sink
18-1	1,733	35.78481659	−78.67285967	Swab	Owen Residence Hall	Inside in dorm room
25-1	1,535	35.77153404	−78.67522001	Swab	Engineering Building I, Centennial campus	Drain in the middle of the floor of the bathroom
29-2	1,452	35.78751407	−78.66981704	Swab	D.H. Hill Jr. Library	Floor 1
7-2	1,381	35.78412031	−78.67101431	Swab	Talley Student Union	Bathroom sink drain
30-2	1,113	35.78824567	−78.67403984	Swab	Nelson Hall	Water fountain