Fig 5. Effect of hydrogen gas inhalation on ROS production.
Tissue samples were prepared from cuffed arteries 7 days after operation. (A) Representative photos of cross-sections of injured femoral artery after DHE staining and fluorescence intensity in intima and media. (B) Representative photos of cross-sections of injured femoral artery after HPF staining and fluorescence intensity in intima and media. The original photos were obtained as 8-bit images (original magnification ×200; scale bar: 100 μm), and data represent relative fluorescence units (RFU). Values are mean ±SEM (n = 18 to 21 for each group). **p<0.01 vs. air group (Con). Hyd; hydrogen group.