Skip to main content
. 2020 Apr 17;15(4):e0231582. doi: 10.1371/journal.pone.0231582

Fig 1. Effect of intervention on plasma cholesterol and development of lipid streaks in aortic roots.

Fig 1

Panel A. Cholesterol levels. ApoE−/− mice fed a HFD for 6 weeks were divided into 5 groups (n = 16/group). One went untreated (Cont). Two groups were given two oral doses of either a low or high dose cholestyramine, 1.5% (Choles L) or 3% (Choles H), respectively. Two groups were given two IV injections of α-def-1, 10 or 30 μg, every other day (α-def-1 L) and (α-def-1 H) respectively. A sixth group was given a modified high fat diet (MFD) for 6 weeks. Blood samples were taken after 6 hours of fasting. Plasma levels of total cholesterol (TCH) were measured. Panel B. Development of lipid streaks. ApoE−/− mice on HFD or MFD for 6 weeks described in Panel A were sacrificed after blood draw. Lesion size in sections from the aortic roots (See Panel C) was measured as described in the Methods section. Results are expressed as the percent of the aortic root circumferences stained by Oil Red O. The mean ± S.D. and p < 0.05 values are shown; n = 16 per group. Panel C. Lipid streaks. Representative sections from the various experimental groups are shown. Scale bars represent 500μm.