Fig 4. Morphology of wild type M. tuberculosis cells and cells from the pknA, pknB or pknA+pknB depletion strains.

4A) Phase contrast microscopy of cells from wild type, pknB, or pknA+pknB depletion strain in the presence (0.25 μg/ml) or absence of ptc. For pknA depletion cells were grown in 0.25 μg/ml of ptc or 20 ng/ml atc. Pictures were taken with a 100x objective. Size bar = 5μM. 4B) Distribution of length measurements of cells of wild type, pknA, pknB and pknA+pknB depletion mutants obtained with ImageJ software. More than 200 cells were counted for each strain. The boxes represent the middle 50% of measurements and the whiskers the minimum to maximum values of the measurements.