Table 3. Primers for single copy gene detection and sub-species-specific analysis.
| Primer name | Target species | Primer sequence 5'-3' | Multiplex product size (bp) | Reference |
| PLC 657 | Trypanozoon | CTT TGT TGA GGA GCT GCA | 324 | [17] |
| PLC 658 | CAC CGC AAA GTC GTT ATT | |||
| SRA 02 | T. b. rhodesiense | AGC CAA AAC CAG TGG GCA | 669 | |
| SRA 03 | TAG CGC TGT CCT GTA GAC GCT | |||
| VSG 651 | Trypanozoon | GAA GAG CCC GTC AAG AAG GTT TG | >1Kb | |
| VSG 651 | TTT TGA GCC TTC CAC AAG CTT GGG | |||
| Primer name | Target species | Primer sequence 5'-3' | Singleplex product size (bp) | Reference |
| TgsGP forward | T. b. gambiense | GCT GCT GTG TTC GGA GAG C | 308 | [34] |
| TgsGP reverse | GCC ATC GTG CTT GCC GCT C |