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. 2014 Aug 28;2(10):813–822. doi: 10.1016/S2213-2600(14)70158-4

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Copyright © 2014 Elsevier Ltd. All rights reserved.

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Replication kinetics of human and camel MERS-CoV in ex-vivo cultures of human bronchus and lung

(A) Thermal inactivation of MERS-CoV at 37°C. MERS-CoV strains were all inactivated at 24 h post incubation, irrespective of initial concentration; with the exception of the highest virus input of NRCE-HKU270. Replication curves of MERS-CoV infection of human bronchial (B) and lung (C) tissues, by individual tissue donor. The horizontal dotted line denotes the limit of detection in the TCID₅₀ assay. MERS-CoV=Middle East respiratory syndrome coronavirus. EMC=human MERS-CoV EMC. AH13=MERS-CoV Dromedary/Al-Hasa-KFU-HKU13/2013. AH19D=MERS-CoV Dromedary/Al-Hasa-KFU-HKU19D/2013. NRCE-HKU270=MERS-CoV Dromedary/Egypt-NRCE-HKU270/2013. TCID₅₀=50% tissue culture infective dose.