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. 2008 Dec 1;39(1):89–100. doi: 10.1111/j.1365-2222.2008.03151.x

Figure 5.

Figure 5

 TSLP+poly(I : C)‐DCs reduced IL‐17 and IFN‐γ double‐producing T cells and increased IL‐17 single‐producing T cells. CD11c+ DCs cultured with TSLP, poly(I : C), a combination of both stimuli, or medium alone were used to prime naïve CD4+ T cells. After 7 days of co‐culture, T cells were restimulated for 6 h with PMA plus ionomycin, and production of indicated T cell‐derived cytokines was determined by intracellular cytokine staining. (a), Dot blots profiles of indicated cytokines. Numbers indicate percent of cells in each quadrant. Data represent one of three independent experiments. (b), Percentage of TNF‐α– IL‐17–, IL‐13 – producing cells. Data represent numbers in (a). (c), Percentage of IL‐17 single‐producing cells (IFN‐γ−IL‐17+), and IFN‐γ and IL‐17 double‐producing cells (IFN‐γ+IL‐17+). Data represents numbers in (a). TSLP, thymic stromal lymphopoietin; DCs, dendritic cells.