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. 2007 Mar 9;37(3):420–433. doi: 10.1111/j.1365-2222.2007.02672.x

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Fig. 6. Effect of diesel exhaust particles (DEP) and mite antigen on HLA‐DR and CD86 expression in cultured nasal epithelial cells (CNEC) from perennial allergic rhinitis (PAR) patients. The effect of DEP and mite antigen on HLA‐DR and CD86 expression on nasal epithelial cells was analysed by flow cytometry after treatment of CNEC with DEP plus purified mite antigen (Der f II) for 5 days, as described in the text. (a) Histogram of a representative sample of a patient with PAR showing up‐regulation of HLA‐DR (left histogram) and CD86 (right histogram) in CNEC after treatment with DEP plus (Derf II) mite antigen (violet full shaded: unstimulated CNEC; green line: after stimulation with DEP plus mite antigen). A shift to the right can be seen after treatment with DEP plus (Derf II) mite antigen as compare with unstimulated CNEC. (b) Results are expressed as the percentage‐positive cells expressing HLA‐DR or CD86. An increase in the percentage positive CNEC expressing HLA‐DR and CD86 can be seen after treatment with DEP plus (Derf II) mite antigen. (n=7); ^** P<0.01.