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. 2020 Mar 25;9:e52786. doi: 10.7554/eLife.52786

Figure 2. Transient expression of the FBP can generate auto-luminescence in a range of plant species.

(A) Luminescence signal superimposed on a bright field image of Solanum lycopersium (tomato) seedlings with an FBP delivered via the AgroBEST protocol. Warmer colors correspond to higher luminescence signal. (B) Luminescence signal superimposed on bright field images of Arabidopsis thaliana seedlings with a functional FBP (left three seedlings) or a broken FBP (right most seedling) delivered via the AgroBEST protocol. Warmer colors correspond to higher luminescence signal. (C) Luminescence signal superimposed on a bright field image of a Dahlia pinnata leaf infiltrated with an FBP. (D) Lit (left) and unlit, long exposure (right) true color images of Catharanthus roseus flowers infiltrated with an FBP. (E) Lit (left) and unlit, long exposure (right) true color images of Rosa rubiginosa flowers infiltrated with an FBP.

Figure 2.

Figure 2—figure supplement 1. DSLR-based imaging produces comparable images of FBP luminescence to CCD-based imaging systems.

Figure 2—figure supplement 1.

Petal infiltrations of C. roseus flowers with an FBP imaged three days post infiltration with either our DSLR-based imaging system (A) or a CCD based imaging system (B).