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. 2020 Apr 17;6(16):eaaz0359. doi: 10.1126/sciadv.aaz0359

Fig. 1. Maternal exercise intervention markedly induces the expression of thermogenic markers in iBAT and ingWAT of offspring at weaning.

Fig. 1

(A and B) Body weight (A) and relative wet weight of iBAT and ingWAT (B) normalized to body weight of female and male M-Ctrl and M-Ex offspring at weaning (n = 6 mice per group). N.S., not significant. (C) Z scores of mRNA levels of different brown fat markers in the iBAT and ingWAT of M-Ctrl and M-Ex offspring at weaning (n = 6 mice per group). Expression was normalized by ΔCt values. (D) Cropped Western blots of UCP1, PRDM16, and PGC-1α in the iBAT (β-tubulin is the loading control) from the E18.5 fetuses of CON and EX maternal mice (n = 6 fetuses per group). (E and F) Cropped Western blots of UCP1 and PRDM16 (β-tubulin is the loading control) from iBAT (E) and ingWAT (F) isolated from female and male mice of M-Ctrl and M-Ex at weaning (n = 6 mice per group). Data are mean ± SEM, and each dot represents one litter. *P < 0.05, **P < 0.01, and ***P < 0.001 by two-tailed unpaired Student’s t test (A to C, E, and F) or one-way analysis of variance (ANOVA) (D).