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. 2020 Apr 17;6(16):eaaz4316. doi: 10.1126/sciadv.aaz4316

Fig. 5. The internalization kinetics of the softest (0% TEOS, 560 kPa) and the stiffest (100% TEOS, 1.18 GPa) SNCs in receptor-mediated uptake by SKOV3 cells.

Fig. 5

(A) Fluorescence live-cell images showing the internalization of the softest and (B) stiffest FA-PEG–modified SNCs by SKOV3 cells and their transport to lysosomes over 60 min; scale bars, 20 μm. Representative time projection images showing the movement of the (C) softest and (D) stiffest SNCs during cellular uptake; tracks of the SNCs are shown in the magnified images of boxed regions. Yellow solid lines indicate the plasma membranes; scale bars, 10 μm. Instantaneous velocity of the (E) softest and (F) stiffest SNCs during stage I and stage II of cellular uptake. MSD analysis of the movement of the (G) softest and (H) stiffest SNCs during stage I and stage II of cellular uptake; insets are magnified graphs. (I) Time taken for the internalization of SNCs. Movements of the SNCs were only recorded for 15 min, as imaging longer than 15 min could lead to fluorescence quenching and focal plane shifting.