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. 2020 Apr 1;9:e51576. doi: 10.7554/eLife.51576

Figure 6. CAV1 high expressing human satellite cells engraft robustly after transplantation in mice.

Figure 6.

(a) Representative immunofluorescence staining images for human specific DYSTROPHIN (scale, 200 µm) within NSG mouse muscle cross-sections in TAs transplanted with CAV1+ and CAV1- human satellite cells. 500 cells were transplanted in each NSG TA. (n = 3, using three separate donors.) (b) Bar graph depicting the number of DYSTROPHIN positive human fibers in mice transplanted with CAV1+ and CAV1- cells. (n = 3, biological replicates) *p<0.05. Data presented as mean ± SEM. (c) Representative immunofluorescence staining images for human PAX7, SPECTRIN, LAMIN A/C and LAMININ within NSG mouse muscle cross-sections in TAs transplanted with CAV1+ and CAV1- human satellite cells (scale, 100 µm). (n = 3, biological replicates). (d) Bar graph represents number of human PAX7+ cells in mice transplanted with both CAV1+ and CAV1- cells. (n = 3, biological replicates) *p<0.05. Data presented as mean ± SEM. (e) Human satellite cells were re-isolated by FACS from mice transplanted with CAV1+ and CAV1- satellite cells.

Figure 6—source data 1. CAV1 high expressing human satellite cells engraft robustly after transplantation in mice.