Figure 2. Difference in T_m for 151 FRESCO-predicted ADHA mutants.

The average of two measurements is given and the standard error. The T_m of wild-type ADHA is 43 °C (set as 0). The 10 stabilizing mutations with a red bar were combined. Melting curves of wild type and the final mutant (M9*) are depicted in Figure 2—figure supplement 1.

Figure 2—figure supplement 1. Melting curves.

Apparent melting temperatures of wild type (in grey) and M9* variant. RFU = relative fluorescence units. M9* displays a first melting peak at 79 °C, second peak at 88 °C. One curve is shown from three technical replicates.