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. 2020 Mar 31;9:e54639. doi: 10.7554/eLife.54639

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© 2020, Aalbers et al

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Figure 7. — (A) Temperature-activity profile using cyclohexanol as substrate. The dashed lines indicate the T_mof the wild type (at 43 °C) and apparent melting temperatures of M9* (78.5 °C and 88 °C) (B) Enzyme activity monitored over time at 37 °C (buffer composition: 50 mM Tris-HCl pH 7.5). Figure 7—figure supplement 1 depicts the enzyme activity over time for 18 days.

Figure 7—figure supplement 1. — (A) Temperature-activity profile using cyclohexanol as substrate. The dashed lines indicate the T_mof the wild type (at 43 °C) and apparent melting temperatures of M9* (78.5 °C and 88 °C) (B) Enzyme activity monitored over time at 37 °C (buffer composition: 50 mM Tris-HCl pH 7.5). Figure 7—figure supplement 1 depicts the enzyme activity over time for 18 days.