Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | NEB 10-beta chemically competent E. coli | New England Biolabshttps://www.neb.com/ | C3019I | |
| Sequence based reagent | pBAD Golden gate vector (N-terminal 6xHis, araC, bla) | This study | Molecular Enzymology Group, University of Groningen | |
| Polymerase | PfuUltra II Hotstart PCR Master Mix | Agilent Technologies https://www.agilent.com/ | 600852 | |
| Commercial assay or kit | Ni chromatography resin | GE Healthcare Life Sciences https://www.gelifesciences.com/ | ||
| Software, algorithm | Rosetta | Rosetta Commons https://www.rosettacommons.org/ | RRID:SCR_015701 | |
| Software, algorithm | FoldX | FoldXhttp://foldxsuite.crg.eu/ | RRID:SCR_008522 | |
| Software, algorithm | YASARA | YASARA Biosciences GmbHhttp://www.yasara.org/ | RRID:SCR_017591 | |
| Software, algorithm | FRESCO scripts | https://groups.google.com/forum/#!forum/fresco-stabilization-of-proteins | ||
| Software, algorithm | GraphPad Prism | GraphPad Prism https://graphpad.com | RRID:SCR_015807 | Version 6 |