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. Author manuscript; available in PMC: 2020 Oct 15.
Published in final edited form as: Cancer Res. 2020 Feb 13;80(8):1693–1706. doi: 10.1158/0008-5472.CAN-19-3466

Figure 6. LIN9 or NEK2 loss potentiates taxane-induced mitotic defects and chromosomal instability.

Figure 6.

MDA-MB-231 sensitive/parental (panels A-D) and resistant (panels E-H) cells were transfected with siNS, siLIN9, or siNEK2, treated for 4 days with DMSO or 6nM paclitaxel, and then stained with DAPI (nuclei), Texas Red-X phalloidin (actin cytoskeleton), and γ-tubulin (centrosomes). The percent of MDA-MB-231 sensitive/parental and resistant cells with (A and E) 3+ centrosomes, (B and F) micronuclei, (C and G) multinucleation, and (D and H) dysmorphic nuclei were counted. Distinct letters above bars indicate significant differences between groups (p<0.05). A minimum of 150 cells were counted per experiment. All data are represented as means ± SD and were repeated in 3 independent experiments in triplicate.