Table 2.
Detection of SARS-CoV-2 in wastewater samples at three WWTPs in Southeast Queensland, Australia.
| Sources of wastewater and sample ID | Types of samples | Sampling date | Sample volume processed | Virus concentration methods and RT-qPCR assays |
|||
|---|---|---|---|---|---|---|---|
| Method A |
Method B |
||||||
| N_Sarbeco | NIID_2019-nCOV | N_Sarbeco | NIID_2019-nCOV | ||||
| PS A1 | Composite grab sample | 20/03/2020 | 200 mL | ND | ND | ND | ND |
| WWTP A1 | Composite autosamplera | 29/03/2020 | 200 mL | ND | ND | ND | ND |
| WWTP A2 | Composite autosamplerb | 30/03/2020 | 200 mL | ND | ND | ND | ND |
| WWTP A3 | Composite autosamplerb | 24/02/2020 | 100 mL | ND | ND | ND | ND |
| WWTP A4 | Composite autosamplera | 29/03/2020 | 100 mL | ND | ND | ND | ND |
| WWTP A5 | Composite autosamplera | 30/03/2020 | 100 mL | ND | ND | ND | ND |
| WWTP A6 | Composite autosamplera | 28/03/2020 | 100 mL | ND | ND | ND | ND |
| WWTP B1 | Composite grab sample | 27/03/2020 | 100 mL | + (~12.0)c | ND | ND | ND |
| WWTP B2 | Composite grab sample | 01/04/2020 | 100 mL | ND | ND | + (~1.90)c | ND |
PS: Pumping station; ND: Not detected
a
A conventional refrigerated autosampler.
b
A submersible in-situ high frequency autosampler.
c
Copies/100 mL of untreated wastewater.