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. 2020 Apr 18;244:108684. doi: 10.1016/j.vetmic.2020.108684

Fig. 1.

Fig. 1

PEDV infection increases autophagy in IPEC-J2 cells. (A, B) Photos of transmission electron microscopy (TEM) shown that the number of autophagosome-like vesicles (double- and single-membrane vesicles containing cytosolic components or sequestered organelles) were significantly higher in the cytoplasm of both the PEDV pandemic strain YC2014 and the classical strain CV777 infected cells than in mock-infected cells. The number of autophagosome-like vesicles in the pandemic strain YC2014 infected cells was significantly higher than the classical strain CV777 infected cells. IPEC-J2 cells were infected at a MOI of 10 for 12 h. *P < 0.05, ***P < 0.001. (C, D) PEDV infection increases the conversion of LC3-I to LC3-II. C, Western blot of PEDV-infected (MOI of 10) or mock-infected IPEC-J2 cells. D, The ratio of LC3-II to β-actin was quantitated by densitometry (n = 3). The ratios of LC3-II to β-actin were both significantly higher in the classical strain CV777 and the pandemic strain YC2014 infected cells than in uninfected cells 12 hpi. The ratios of LC3-II to β-actin in the pandemic strain YC2014 infected cells were significantly higher than the classical strain CV777 infected cells. Data were expressed as the mean ± SD of three independent experiments and were analyzed by one-way ANOVA. **P < 0.01, *P < 0.05, ***P < 0.001. (E) Both GFP-LC3 and RFP-LC3-labeled puncta were detectable in IPEC-J2 cells after PEDV YC2014 strain and CV777 strain infection.