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. 2020 Apr 18;244:108684. doi: 10.1016/j.vetmic.2020.108684

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Fig. 3 — PEDV replication is required for PEDV-induced autophagy. (A) IFA of replication-competent PEDV or replication-incompetent PEDV (UV-treated) in IPEC-J2 cells. (B) Western blot showing the levels of LC3-I and LC3-II in IPEC-J2 cells 24 hpi with PEDV and UV-treated PEDV. (C) The ratio of LC3-II to β-actin was quantitated by densitometry (n = 3). Data were expressed as the mean ± SD of three independent experiments and were analyzed by one-way ANOVA. ***P < 0.001. (D) Western blot analysis of the eukaryotic expressed the structural proteins. (E) Western blot analysis of LC3-I and LC3-II in IPEC-J2 cells expressing structural proteins. (F) The ratio of LC3-II to β-actin was quantitated by densitometry (n = 3). Data were expressed as the mean ± SD of three independent experiments and were analyzed by one-way ANOVA. Same letter (a) indicates no significant difference between the groups.