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. 2020 Apr 18;244:108684. doi: 10.1016/j.vetmic.2020.108684

Fig. 5.

Fig. 5

Examination of nsps-induced autophagy in IPEC-J2 cells. (A) Western blot analysis of the eukaryotic expression of the nsps. (B, C) Confocal immunofluorescence microscopy analysis of autophagosomes-like vesicles in IPEC-J2 cells expressing nsps. Nsp6 or ORF3 expression causes an increase of autophagosome-like vesicles, but the expression of the other nsps does not.