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. 2020 Apr 18;244:108684. doi: 10.1016/j.vetmic.2020.108684

Fig. 6.

Fig. 6

Nsp6-induced autophagy occurs via the PI3K/Akt/mTOR signaling pathway. (A) Western blot detection of cytokines and their phosphorylation levels in nsp6-expressing IPEC-J2 cells, rapamycin-treated IPEC-J2 cells or mock-treated IPEC-J2 cells. (B) The ratio of cytokines to β-actin as determined by densitometry (n = 3). Data were expressed as the mean ± SD of three independent experiments and were analyzed by one-way ANOVA. *P  <  0.05, **P < 0.01. (C, D) The inhibition efficiency of Akt-specific inhibitor GSK690693 in IPEC-J2 cells as determined by western blot 24 hpi (n = 3). Data were expressed as the mean ± SD of three independent experiments and were analyzed by one-way ANOVA. **P <  0.01, ***P <  0.001. (E) PEDV titers were increased in cells with Akt-specific inhibitor GSK690693 compared to the titers in mock cells (n=3). Data are presented as the mean ± SD of three independent experiments. *P <  0.05, **P <  0.01.