Fig. 4.

Confirmation of the expression levels of the IGF1 and NXPE2 proteins in patients with diffuse-type gastric cancer (GC). a Comparison of images of IGF1 protein expression generated via tissue microarray analysis between diffuse-type patients with the core diffuse (COD) and intestinal-like (INT) subtypes. b Comparison of images of NXPE2 protein expression generated via tissue microarray analysis between diffuse-type patients with the COD and INT subtypes. c Comparative analysis of the protein expression levels of IGF1 and NXPE2 between diffuse-type GC patients with the INT, COD, and COD with poorly cohesive carcinoma (PCC) subtypes. Each bar represents the mean ± standard deviation of three independent experiments. d Comparison of the expression levels of the IGF1 and NXPE2 proteins between the histological non-PCC and PCC subclasses. IGF1 protein expression levels showed a statistically significant correlation with the histological subtypes. r values were obtained by biserial correlation tests and P values were obtained by two-sample t tests. *P < 0.05, **P < 0.01, ***P < 0.001