Fig. 7.

Summary of the anticancer effect of huATN-658 + Zometa combination. (1) The combination treatment reduces tumor cell proliferation and invasion in vitro, which suggests that such treatment strategy may also reduce tumor cell growth and metastasis to different organ in vivo. As shown in this study, the huATN-658 monotherapy significantly reduces mammary tumor volume in immunocompromised mice in vivo. However, further studies are warranted to assess whether huATN-658 in combination with Zometa treatment could reduce primary tumor volume and metastasis using appropriate models. (2) In vivo evidence obtained from intratibial injection of breast cancer cells revealed that the combination treatment reduces tumor cell growth and skeletal lesion. In addition, the combination treatment reduces the number of osteoclasts, which may also help to decrease the skeletal lesions