Skip to main content
. 2020 Apr 17;11(4):240. doi: 10.1038/s41419-020-2443-y

Fig. 3. The RNAPII elongation rate within the Spo11 gene is modulated in meiosis.

Fig. 3

a Workflow used for detection of EU-labeled nascent RNAs. P14 and P18 mice were treated by intraperitoneal injection of EU (300 μg/g). EU-labeled newly synthesized RNAs were collected from testes 2 h after injection, biotinilated and captured by streptavidin magnetic beads for analysis. b qPCR analysis of nascent Spo11 and Sycp1 transcripts from P14 and P18 mouse testis. The graph represents the ratio between distal and proximal intron of EU-labeled RNA. (mean ± SD; n = 3; ∗∗P ≤ 0.01, unpaired t test). c, d qPCR analysis of different regions of the nascent Spo11 pre-mRNA corresponding in P14 (c) and P18 (d) mouse testis (mean ± SD; n = 3; ∗P ≤ 0.05, ∗∗P ≤ 0.01, ∗∗∗P ≤ 0.001 and ∗∗∗∗P ≤ 0.0001 related to int1, one-way ANOVA, Bonferroni’s multiple comparisons test). e, f ChIP assays of serine 2-phosphorylated (d) or total (e) RNAPII in the exon 2 region of Spo11 in testicular germ cells at P14 and P18. The bar graph shows qPCR signals expressed as enrichment relative to IgG (mean ± SD, n = 3; *P ≤ 0.05, unpaired t test).