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. 2020 Apr 17;11(4):239. doi: 10.1038/s41419-020-2431-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Hematoxylin and eosin staining of renal cell carcinoma (RCC) specimens and adjacent normal renal tissues. b Immunohistochemical staining of caspase-1 in RCC specimens and adjacent normal renal tissues. c Immunohistochemical analysis of IL-1β in RCC tissues and adjacent normal renal tissues. d Western blot analysis of expression levels of caspase-1 and IL-1β in RCC tissues and surrounding normal regions. e Relative caspase-1 and IL-1β protein levels in HK-2 cells (human renal tubule epithelial cell line), and five RCC cell lines (A498, ACHN, CAKI-1, 786-O, and OSRC-2) were analyzed using western blot analysis. Results are shown as means ± SD. *P < 0.05, relative to the HK-2 cell line.