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. 2020 Apr 17;11(4):239. doi: 10.1038/s41419-020-2431-2

Fig. 4. Pyroptosis activation mediated BRD4 inhibition-induced suppression of cell proliferation and EMT in RCC cells.

Fig. 4

786-O cells were pre-treated with 50 μM Ac-YVAD-CMK or 50 μM Z-DEVD-FMK for 24 h, then transfected with siBRD4; ACHN cells were pre-treated with 50 μM Ac-YVAD-CMK or 50 μM Z-DEVD-FMK for 24 h, then treated with JQ1. a, d Cell proliferation of 786-O and ACHN cells was measured using CCK8 assay. b, c, e, f Protein levels of E-cadherin and vimentin were measured by western blotting and normalized to GAPDH. Experiments were performed in triplicate. *P < 0.05 vs. control; #P < 0.05 vs. si-BRD4 or JQ1; N.S. P > 0.05 vs. si-BRD4 or JQ1.