Fig. 5. JQ1 impaired tumor growth and EMT progression via pyroptosis in mice.

ACHN cells were subcutaneously inoculated into the left flanks of nude mice. Before injection, the cells were pretreated with LPS through the methods mention above, then the mice were treated with vehicle, JQ1, or combination of Ac-YVAD-CMK and JQ1. a, b Immunohistochemical staining for ki-67 in xenografted tumors. Image-Pro Plus software was used to determine the average IOD. c–e Western blot analysis of BRD4 and pyroptosis-related proteins, such as caspase-1 and IL-1β, and EMT-associated proteins, such as E-cadherin and vimentin, in xenografted specimens. *P < 0.05 vs. vehicle; ^#P < 0.05 vs. JQ1; N.S. P > 0.05 vs. JQ1.