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. 2020 Apr 17;11(4):239. doi: 10.1038/s41419-020-2431-2

Fig. 6. BRD4 inhibition regulated NLRP3-induced pyroptosis, cell proliferation, and EMT in RCC cells.

Fig. 6

a, b 786-O and ACHN cells were transfected with NLRP3 plasmid or control plasmid. NLRP3 levels were detected by western blotting. *P < 0.05 vs. control plasmid. c–h 786-O cells were transfected with si-NLRP3 or si-NC, then transfected with si-BRD4. ACHN cells were pre-treated with 10 μM MCC950 for 2 h, then treated with JQ1. c, d Western blot analysis of NLRP3 protein levels, and bar graphs from three independent experiments. e, f Western blot analysis of caspase-1, IL-1β and GSDMD protein levels in the indicated groups. g, h Western blot analysis of E-cadherin and Vimentin in the indicated groups. Experiments were performed in triplicate. *P < 0.05 vs. control; #P < 0.05 vs. si-BRD4 or JQ1; &P < 0.05 vs. si-NLRP3 or MCC950; Le-NC, control plasmid; Le-NLRP3, NLRP3 plasmid.